plasmidfu tet o hoct4 Search Results


s2805  (Selleck Chemicals)
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Selleck Chemicals s2805
S2805, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a 83 01 selleck  (Selleck Chemicals)
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Selleck Chemicals a 83 01 selleck
A 83 01 Selleck, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sb431542 selleck  (Selleck Chemicals)
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Figure 2. CDK8 was a barrier of MEF-XEN reprogramming (A) CDK8 activity inhibition assays in vitro for SEL120-34A HCl (control), MSC2530818, E-616452, and A-83-01. (B) qPCR of Sox17, Gata4, Sall4, and Foxa2 in samples with MEF medium, base, CFAE+8+DMSO/BI/MSC, and CFAE+6 treatment and base control for 12 days, respectively (C, CHIR99021; F, forskolin; A, AM580; E, EPZ04777; 6, E-616452; 8, A-83-01; S, <t>SB431542;</t> L, LY364947). Base, XEN basal medium. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 between CFAE+8+DMSO and indicated samples. Numbers in brackets are the concentrations (mM) of each small molecule.
Sb431542 Selleck, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmidfu+tet+o+hoct4/SB431542/pm37235474-162-17-18
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resource source identifier dexamethasone selleck  (Selleck Chemicals)
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Selleck Chemicals resource source identifier dexamethasone selleck
Figure 2. CDK8 was a barrier of MEF-XEN reprogramming (A) CDK8 activity inhibition assays in vitro for SEL120-34A HCl (control), MSC2530818, E-616452, and A-83-01. (B) qPCR of Sox17, Gata4, Sall4, and Foxa2 in samples with MEF medium, base, CFAE+8+DMSO/BI/MSC, and CFAE+6 treatment and base control for 12 days, respectively (C, CHIR99021; F, forskolin; A, AM580; E, EPZ04777; 6, E-616452; 8, A-83-01; S, <t>SB431542;</t> L, LY364947). Base, XEN basal medium. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 between CFAE+8+DMSO and indicated samples. Numbers in brackets are the concentrations (mM) of each small molecule.
Resource Source Identifier Dexamethasone Selleck, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmidplko 1 trc cloning vector  (Addgene inc)
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Addgene inc plasmidplko 1 trc cloning vector
Figure 2. CDK8 was a barrier of MEF-XEN reprogramming (A) CDK8 activity inhibition assays in vitro for SEL120-34A HCl (control), MSC2530818, E-616452, and A-83-01. (B) qPCR of Sox17, Gata4, Sall4, and Foxa2 in samples with MEF medium, base, CFAE+8+DMSO/BI/MSC, and CFAE+6 treatment and base control for 12 days, respectively (C, CHIR99021; F, forskolin; A, AM580; E, EPZ04777; 6, E-616452; 8, A-83-01; S, <t>SB431542;</t> L, LY364947). Base, XEN basal medium. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 between CFAE+8+DMSO and indicated samples. Numbers in brackets are the concentrations (mM) of each small molecule.
Plasmidplko 1 Trc Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. CDK8 was a barrier of MEF-XEN reprogramming (A) CDK8 activity inhibition assays in vitro for SEL120-34A HCl (control), MSC2530818, E-616452, and A-83-01. (B) qPCR of Sox17, Gata4, Sall4, and Foxa2 in samples with MEF medium, base, CFAE+8+DMSO/BI/MSC, and CFAE+6 treatment and base control for 12 days, respectively (C, CHIR99021; F, forskolin; A, AM580; E, EPZ04777; 6, E-616452; 8, A-83-01; S, SB431542; L, LY364947). Base, XEN basal medium. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 between CFAE+8+DMSO and indicated samples. Numbers in brackets are the concentrations (mM) of each small molecule.

Journal: Cell reports

Article Title: Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems.

doi: 10.1016/j.celrep.2023.112566

Figure Lengend Snippet: Figure 2. CDK8 was a barrier of MEF-XEN reprogramming (A) CDK8 activity inhibition assays in vitro for SEL120-34A HCl (control), MSC2530818, E-616452, and A-83-01. (B) qPCR of Sox17, Gata4, Sall4, and Foxa2 in samples with MEF medium, base, CFAE+8+DMSO/BI/MSC, and CFAE+6 treatment and base control for 12 days, respectively (C, CHIR99021; F, forskolin; A, AM580; E, EPZ04777; 6, E-616452; 8, A-83-01; S, SB431542; L, LY364947). Base, XEN basal medium. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 between CFAE+8+DMSO and indicated samples. Numbers in brackets are the concentrations (mM) of each small molecule.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dexamethasone Selleck Cat# S5956; CAS: 2392-39-4 A-83-01 Selleck Cat# S7692; CAS: 909910-43-6 SB431542 Selleck Cat# S1067; CAS: 301836-41-9 LY364947 Selleck Cat# S2805; CAS: 396129-53-6 Y-27632 Selleck Cat# S6390; CAS: 146986-50-7 Hydrocortisone (cortisol) TargetMol Cat# T1614; CAS 50-23-7 Triiodothyronine TargetMol Cat# T29011; CAS 31135-55-4 Rosiglitazone TargetMol Cat# T0334; CAS 122320-73-4 BI-1347 TargetMol Cat# T5405; CAS 2163056-91-3 MSC2530818 TargetMol Cat# TQ0266; CAS 1883423-59-3 Kinase inhibitor library MedChemExpress Cat# HY-L00 Critical commercial assays EndoFree Maxi Plasmid Kit TIANGEN Cat# DP117 TIANprep Rapid Mini Plasmid Kit TIANGEN Cat# DP105 Universal DNA Purification Kit TIANGEN Cat# DP214-03 TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Cat# AT311-02 EasyPure RNA Kit TransGen Cat# ER101-01 TransStart FastPfu Fly DNA Polymerase TransGen Cat# AP231-02 pEASY-Uni Seamless Cloning and Assembly Kit TransGen Cat# CU101-02 KAPA HiFi PCR Kits KAPA BIOSYSTEMS Cat# KK2602 TruePrep DNA Library Prep Kit V2 for Illumina Vazyme Cat# TD502-01 Deposited data ATAC-Seq This paper GEO: GSE227566 Drug-seq2 This paper GEO: GSE227568 RNA-seq This paper GEO: GSE227569 Mouse reference genome mm10 UCSC https://hgdownload.soe.ucsc.edu/ goldenPath/mm10/bigZips/ Experimental models: Cell lines Mouse: mESC TT2 RIKEN BioResource Center Stock No.: AES0014 Experimental models: Organisms/strains Mouse: B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J The Jackson Labratory RRID:IMSR_JAX:004654 Oligonucleotides Primers for quantitative real-time PCR, see Table S1 This paper N/A Recombinant DNA FU-tet-o-hOct4 (Maherali et al., 2008)37 Addgene PlasmidFU-tet-o- hOct4 Cat# 19778 FU-tet-o-GFP This paper N/A FU-tet-o-mCdk8-2HA This paper N/A FU-tet-o-mHnf4a This paper N/A FU-tet-o-mFoxa3 This paper N/A FU-tet-o-hCDK8 This paper N/A FU-tet-o-hCDK8-DFG This paper N/A FU-tet-o-hCDK8-15AA This paper N/A FU-tet-o-hCDK8-NMG This paper N/A pLKO.1-TRC cloning vector (Moffat et al., 2006)38 Addgene PlasmidpLKO.1-TRC cloning vector Cat# 10878 pLKO.1-scramble This paper N/A (Continued on next page) Cell Reports 42, 112566, June 27, 2023 17

Techniques: Activity Assay, Inhibition, In Vitro, Control